Clinical effect of azithromycin adjuvant therapy in children with bronchiolitis: a systematic review and Meta analysis / 中国当代儿科杂志

OBJECTIVE@#To systematically evaluate the clinical effect of azithromycin (AZM) adjuvant therapy in children with bronchiolitis.@*METHODS@#Related databases were searched for randomized controlled trials (RCTs) on AZM adjuvant therapy in children with bronchiolitis published up to February 17, 2019. RevMan 5.3 was used to perform the Meta analysis.@*RESULTS@#A total of 14 RCTs were included, with 667 children in the intervention group and 651 in the control group. The pooled effect size showed that in the children with bronchiolitis, AZM adjuvant therapy did not shorten the length of hospital stay (MD=-0.29, 95%CI: -0.62 to 0.04, P=0.08) or oxygen supply time (MD=-0.33, 95%CI: -0.73 to 0.07, P=0.10), while it significantly shortened the time to the relief of wheezing (MD=-1.00, 95%CI: -1.72 to -0.28, P=0.007) and cough (MD=-0.48, 95%CI: -0.67 to -0.29, P<0.00001). The analysis of bacterial colonization revealed that AZM therapy significantly reduced the detection rates of Streptococcus pneumoniae (OR=0.24, 95%CI: 0.11-0.54, P=0.0006), Haemophilus (OR=0.28, 95%CI: 0.14-0.55, P=0.0002), and Moraxella catarrhalis (OR=0.21, 95%CI: 0.11-0.40, P<0.00001) in the nasopharyngeal region.@*CONCLUSIONS@#AZM adjuvant therapy can reduce the time to the relief of wheezing and cough in children with bronchiolitis, but it has no marked effect on the length of hospital stay and oxygen supply time.

Training of medical students' critical thinking ability in pathophysiology teaching / 中华医学教育探索杂志

Carrying out the training of critical thinking in pathophysiology teaching is appropriate, and the medical students critical thinking ability can be achieved via construction of the awareness, and diverse teaching methods which include questioning, exploration and discussion.

Pharmacokinetics study of compound xueshuantong soft capsules / 中国中药杂志

<p><b>OBJECTIVE</b>To establish a RP-HPLC method for determination of the content of ginsenoside Rg1 in the rabbits aqueous humor, blood and ocular tissues, which were given intragastric administration with the co-xueshuantong soft capsules. The drug concentration in rabbits at different times after oral administration has been determined and the pharmacokinetics characteristics has been researched.</p><p><b>METHOD</b>The compound xueshuantong soft capsules were administrated to the healthy New Zealand rabbits by gavage (10 mg x kg(-1) per rabbit). The concentration of Ginseng Rg1 in aqueous humor, blood and ocular tissues at different time was determined by RP-HPLC.</p><p><b>RESULT</b>RP-HPLC can be established for the determination of ginsenoside Rg1 in the rabbits aqueous humor, blood, ocular tissue. The calibration of curves was linear within the range of 7.60-152.0 mg x L(-1) (r = 0.999 6) for ginsenoside Rg1 in aqueous humor and the calibration of curves were linear within the range of 10.35-103, 50 mg x L(-1) (r = 0.999 8) for ginsenoside Rg1 in blood. Determination of the recovery rate to meet the requirements.</p><p><b>CONCLUSION</b>The ginsenosides Rg1 could transmit the blood-ocular barrier into the eyes and reach a certain concentration. The research provides a theoretical and experimental basis for the systemic administration of compound Xueshuantong to treat eye diseases.</p>

Effects of morphine on the development of chick embryos / 中国应用生理学杂志

<p><b>AIM</b>To investigate the effect of morphine on fetal movement, heart rate, hatch weight, hatch days and hatch rate.</p><p><b>METHODS</b>Morphine was injected into airspace of eggs and fetal movement, heart rate, hatch weight, hatch days and hatch rates were recorded.</p><p><b>RESULTS</b>Hatch days were shorter, hatch rates were lower and some chicks became motor disorder for morphine. Chicks with morphine exposure 20 mg/kg from E 12 to E 16 had highest hatch rate and lowest disable rate. Morphine reduced fetal movement, increased heart rate (P < 0.05).</p><p><b>CONCLUSION</b>The development of chick embryo is impaired by morphine exposure and the magnitude of these effects depends on the drug dose and the length of time that the developing organism is exposed to morphine.</p>

Teaching experience from the network-aided instruction system for pathophysiology / 中华医学教育探索杂志

As the expansion of classroom teaching,the emerging network-aided instruction could provid novel methods to improve pathophysiological teaching.With the methodological superiority of network and information technology,the network-aided pathophysiological teaching protocol should be well designed to attract learning interest and improve comprehensive abilities of the students.

Effects of curcumin on solid tumor and acute myeloid leukemic cells / 第三军医大学学报

Objective To investigate the effects of curcumin on the proliferation, cell cycle distribution, and apoptosis of the acute myeloid leukemic cell line HL 60 and the hepatocarcinoma cell line QGY. Methods MTT method was used to detect the biological activities of curcumin at different concentrations and at different time. The cell cycle distribution was analyzed by flow cytometry, and changes of the cellular ultrastructure were observed by electronic microscopy. Results Curcumin could inhabit the growth of HL 60 and QGY in dose and time dependent manners. IC50 values of curcumin at 72 h to HL 60 and QGY were 24.8 ?mol/L and 49.5 ?mol/L, respectively. The cell growth of HL 60 was arrested at S and G 2/M stages (apoptosis peak: 8.65%), and that of QGY was arrested at S stage (apoptosis peak: 10.84%). Curcumin could lead to fat degeneration in HL 60 cells and cell degeneration and necrosis of QGY cells, resulting in apoptosis of QGY cells. Conclusion Curcumin has inhibitory effect on the growth of HL 60 cells through its anti proliferation and on QGY cells through the induction of apoptosis of QGY cells.

Effects of curcumin on proliferation and apoptosis in human hepatic cells / 中华肝脏病杂志

<p><b>OBJECTIVE</b>To investigate the effect of curcumin on the proliferation, cell cycle distribution and apoptosis of hepatocarcinoma cell line QGY.</p><p><b>METHODS</b>The MTT method was used to assay the biologic activities of curcumin in different times and different doses. The cell cycle distribution was detected by flow cytometic analysis. The cell ultrastructure was observed by electronic microscopy.</p><p><b>RESULTS</b>Curcumin could inhibit effectively QGY in a dose- and time- dependent manner. IC(50) of curcumin to QGY was 49.50 micromol/L in 72 hours. The cell growth was arrested at S stage. Curcumin could lead to the degeneration, necrosis, and apoptosis.</p><p><b>CONCLUSIONS</b>Curcumin can interrupt the cell cycle and has a role in cytotoxicity, antiproliferation and inducing apoptosis of QGY.</p>